Pankaj Losan Sharma

4 Questions 1 Answers 0 Followers

Questions related from Pankaj Losan Sharma

Can anyone suggest protocols for successful conjugation of Ecoli (TP003) with bacteria such as bacillus and Burkholderia for successful recombination?

I have tried to perform conjugation of E coli having GUS transposon(TP003) with bacillus , Burkholder strains, what I have observed that after mixing both the antibiotic selective strains..with E...

03 March 2019 1,918 1 View

How can I able to perform RNA sequencing of a specific pathogenic bacteria from plant?

I am working on a pathogenic bacteria , i want to analyze its gene expression by RNA seq, but the main obstacle is that I want to study the gene expression of these bacteria while it is present on...

06 June 2016 7,487 2 View

What should be the concentartion of genomic DNA for whole genome de novo sequencing of a bacteria?

What should be the quantity and concentration of genomic DNA we should use for whole genome de novo sequencing (next generation sequencing) of a bacterial sample having size around 6MB?

10 October 2014 3,053 4 View

Which is the best method to quantify concentration of DNA?

There are different ways to calculate or to find out concentration of genomic DNA or any DNA sample . I want to know which is the best method and why?

09 September 2014 495 3 View