Pankaj Singh

3 Questions 3 Answers 0 Followers

Questions related from Pankaj Singh

How to upload the multiple sequence alignment file in primer explorer v5?

I wish to design a common LAMP primer for multiple sequences by primer explorer v 5. I tried to upload multiple files in software like clustal omega alignment, Notepad file with multiple sequence...

14 July 2023 4,713 2 View

How would you explain this type amplification curves from clinical samples?

We are doing Coronavirus testing by real time PCR, targeting E gene (FAM), HKU (VIC) and Actin (ROX) gene. Real time PCR mater mix (20 ul) includes: Taqman Fast virus 1 step master mix (5ul),...

24 September 2020 6,005 3 View

We are growing primary human nasal epithelial cells in BEGM media but cells are not attaching or growing after trypsinisation?

We are growing primary human nasal epithelial cells in BEGM media but cells are not attaching or growing after trypsinisation? What may be the reason?

07 November 2017 5,931 1 View