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Questions related from Panchsheela Nogia
I have to isolate RNA from various types of callus samples. Is it possible that first I grind the callus tissue in liquid nitrogen and then store the powdered form in centrifuge tubes at -80 and...
24 September 2019 3,354 5 View
Here I am using recombinant genomic DNA as template. I have transferred the gene via Agrobacterium mediated co-culture method in Tobacco and then I have isolated genomic DNA from transformed leave...
04 August 2017 7,231 2 View
Basically, I am trying to extract total proteins (all the possible type of proteins including soluble/cytosolic and membrane-associated proteins or may be the protein is expressed inside the...
03 February 2017 1,978 2 View
I have tried transforming A. thaliana and N. tabacum plants using Agrobacterium mediated co-cultivation/culture method but in case of A. thaliana, I could not get any callus after agroinfection...
19 October 2016 5,028 18 View
For GFP localization study by confocal microscopy, I need to prepare a permanent slide of the protoplast samples which are prepared from the agroinfiltrated leaves of Nicotiana benthamiana. Is...
22 July 2016 8,173 7 View
Can anyone suggest me how to avoid fungal infection on regeneration media while doing agrobacterium mediated transformation of arabidopsis leaves by co culture method? For agro removal i prefer to...
18 February 2015 6,428 18 View
What are the important facts/requirements that we need to focus while growing A.thaliana in lab conditions?
11 June 2013 7,151 3 View
In this vector our gene will be under the control of lac promoter, so will it be active in plant system? Anybody have experience working with this vector?
24 May 2013 4,679 9 View
