Pamela Cayuleo

3 Questions 3 Answers 0 Followers

Questions related from Pamela Cayuleo

Is there an alternative method for protein extraction using TRIzol?

I need to extract proteins from cells suspended in TRIzol, but I do not have guanidine hydrochloride in the laboratory. I was wondering if there is a similar reagent or an alternative protocol...

1 weeks ago 9,792 3 View

Why does the Bradford assay for protein quantification in human serum yield negative absorbance values?

I performed several Bradford assays to quantify proteins in human serum, but the absorbance of undiluted serum (with and without protease inhibitors) consistently showed negative values or values...

5 weeks ago 2,649 3 View

¿Por qué la absorbancia de cuantificación por Bradford de proteínas de suero humano da un valor negativo?

Hemos hecho varios ensayos de cuantificación por Bradford, donde la absorbancia de suero humano puro (tanto con inhibidores como sin inhibidores de proteasas) da un valor negativo o menor que el...

5 weeks ago 4,301 2 View