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Questions related from Pallabi Sengupta
I am currently working with a compound which is expected to bind G-quadruplex structure in c-MYC promoter. While I observed very poor affinity between c-MYC quadruplex and the compound in vitro in...
06 August 2018 5,926 1 View
I have screened more than 50 compounds from a chemical library containing 5 lakh molecules to test its selectivity for specific oncogenic G-quadruplex DNA structures. The in silico study reveals...
26 April 2018 5,724 3 View
I had been trying DMS (Dimethyl sulfate) footprinting experiment to elucidate quadruplex folding topology of the putative quadruplex forming sequences. First time: The oligos were 6-FAM labelled...
02 May 2017 4,315 6 View
We are trying to clone a number of gene promoters into a promoter-less luciferase vector and performed PCR reaction using HeLa genomic DNA (250-800 ng). PCR reactions are successful for two gene...
27 January 2017 5,525 10 View
We are attempting to perform DMS (Dimethyl sulphate) footprinting with 6-FAM labelled oligonucleotide sequence. We are confused about the labelling of the DNA sequence by 6-FAM. Do we need to...
02 November 2016 2,665 4 View
I have loaded the FASTA sequence of the gene. I know the ORF. Using different server for promoter prediction I have come up with multiple predicted promoter sequences in the gene. Is there any...
16 August 2016 5,547 2 View
I have performed RT (Reverse transcription) PCR and run agarose gel for densitometric analyses. I need to calculate the concentration of the product from the band intensity only. I was considering...
06 August 2016 9,501 3 View
I am working with ~6000 bp plasmid DNA, which contains the promoter of my interest. This promoter has a cis-element, which forms non-canonical DNA structures in cells, which I have already proved....
01 January 1970 7,819 1 View
We have recently performed DMS (dimethyl sulphate) footprinting experiment to understand the folding topology of the quadruplex forming sequence (90 nt). The sequence is labelled with 6-FAM at its...
01 January 1970 8,063 3 View
I am looking for designing crRNA against the putative G-quadruplex forming motifs. Taking account of the criteria for a good crRNA, web-based design gives crRNA sequences that don't overlap the...
01 January 1970 8,966 1 View
We performed DMS footprinting of a 90 nt sequence. The left most lane is A+G ladder. The 2nd and 3rd lane denote DMS reactions in different conditions which are consistent with our hypotheses. The...
01 January 1970 456 0 View
Crystal Violet is a popular DNA binding dye. It is widely known that fluorescence emission of Crystal Violet (CV) is significantly enhanced upon titrations with anti-parallel G-quadruplex DNA....
01 January 1970 6,860 0 View
