Pablo Rougerie

5 Questions 4 Answers 0 Followers

Questions related from Pablo Rougerie

Plasma membrane dye for epithelial cells?

Hi everyone, i'm looking for an efficient dye to stain the plasma membranes within an epithelial monolayer (MDCK). I tried the FM1-43 but I haven't been convinced with the result. I need to have...

28 July 2016 6,611 1 View

Tips for culture of MDCK cells?

I'd like some tips about the culture of MDCK cells (NBL-2, so it's the parental lineage if I'm correct). Mostly 1. what medium to use? 2. For how many passages can I use the culture? 3. When...

23 December 2015 3,003 2 View

Is it possible to recycle a transwell insert?

Hi all, I am planning to initiate a migration study that will make intensive use of transwell-like culture inserts of various pore size. However, given the high cost that would represent buying...

13 May 2015 2,105 6 View

Does someone have an epithelial cell line easy to cultivate and transfect and form a nice epithelial sheet?

I'm planning to perform some rapid preliminary experiments on epithelial sheets, such as staining for markers of baso-apical polarity and wound healing assay. Ideally I'd like a cell line that can...

15 April 2015 5,264 3 View

Best kit for exosome isolation: Life Technologies, Exoquick or Exo-spin?

I would like to isolate exosomes from a culture of macrophages (RAW) and 3 available exosome purification kits came up: the Exosome Isolation kit (Life Technologies), Exoquick (System Bioscience)...

15 October 2013 5,445 64 View