Pablo José Palma Cancino

4 Questions 11 Answers 0 Followers

Questions related from Pablo José Palma Cancino

How to concentrate a PCR product before sequencing?

Hi everybody, I'm planning to sequence several phytoplasma strains by doing a nested PCR and cloning the resulting amplicons. Standard phytoplasma universal primers pairs will be used (P1/P7...

09 November 2020 2,529 4 View

Adonidia or Veitchia?

Hi, I'm a plant pathologist (not a very good plant biologist I'm afraid) currently struggling. I was studying the spread of a disease on Manila palms (Adonidia merrillii) when I noticed some...

27 August 2019 2,613 8 View

Can you please help me identifying this palm?

Hi, I'm studying phytoplasma related-diseases on palms and for proper description of a syndrome recently detected, I need to identify this palm to the species level. If more photos are needed of...

25 July 2019 5,536 15 View

What's the propper way to analise with PCR an artificial feeding medium (sucrose at 15%) for a planthopper for the presence of phytoplasma DNA?

After the insect feeds, do you have to threat the medium in any way (use an extraction protocol)? can you analyse it directly (using the medium as template)? I'll be using a real-time PCR protocol...

30 August 2017 8,203 1 View