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Questions related from P. W. Noyce
Do I use a specific gene primer in the RT-qPCR workflow to isolate only this transcript sequences for the PCR step. Please note I am not talking about DGE analysis but only transcripts/genes from...
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I have 2 plant samples at very different stages of development- called a= (pre flowering) and b =(at flowering). If I do RNA seq on both samples to get the transcripts and then do DGE analysis...
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About 60% of lncRNA have a poly-A tail, as does mRNA. I wish to isolate only mRNA (by Qiagen kit). How do I get rid of the lncRNA or know that the transcripts I end up with are mRNA only?
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I am storing tissue samples in Farmers Solution (ethanol + acetic acid) for later RNA extraction then RNA seq.
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