Oumed G. M.Amin

4 Questions 14 Answers 0 Followers

Questions related from Oumed G. M.Amin

What is the best way to check aligned sequences for stop codons and how to get rid of them?

I'm trying to do a multiple alignment of coding sequences for a protein I'm interested in, using Mega 5.05. Following the advice in Barry Hall's book, I'm trying to align the sequences using...

10 October 2013 9,232 0 View

Could anyone tell me how to calculate nucleotide sequence similarity and identity?

We did a molecular survey and want to find out the similarity and identity among the amino acid sequences, so that we can interpret our sequence data.

10 October 2013 8,114 23 View

Can anyone explain how to examine the quality of aligned nucleotide sequences?

I am writing an article about a molecular survey on viral poultry diseases. I want to compare several sequences belonging to the same virus but different in pathogenicity and at molecular level so...

10 October 2013 4,419 9 View

Can anyone tell when and how to trim sequences if needed before alignment and or before phylogram building?

We obtained sequences of IBD virus and want to align them with published similar sequences. Sometimes only complete sequences available for a particular isolate in Genbank while I need only VP2...

10 October 2013 1,117 0 View