Oualid abdelkader Bellag

3 Questions 10 Answers 0 Followers

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What type of ends, Mutazyme II polymerase generate?

Hi everyone, I have an epPCR using Genemorph II. I want to clone the epPCR product into a pBAD TOPO plasmid. I wonder if mutazyme II creates A' overhang or I need to do it in another...

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Can a disulfide enter and exit the cell?

Hi everyone, I am doing an enzyme assay using a 6'- thio-glucose bounded to a fluorescent substrate. I have to make sure that the thio-glucose doesn't dimmerize with another substrate giving a...

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Can I use pLySs or pLysE plasmid to transform my gene of interest?

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