Otávio Bravim

3 Questions 3 Answers 0 Followers

Questions related from Otávio Bravim

In Saccharonyces cerevisiae, the fatty acid purification removes the CoA?

I need to detect an oxoacyl in GC MS, but I wonder if the purification protocol of fatty acid described by Murad et al in the article Mass spectrometry characterisation of fatty acids from...

07 July 2016 6,948 0 View

Does anyone knows a purification protocol of Malonyl-CoA in Saccharomyces cerevisiae?

I need to know if an enzyme I added is working by spectometry of Malonyl-CoA, but I do not know how to purify it, does anyone?

11 November 2015 2,252 5 View

In a sequence I have, the promoter contains the restriction enzyme sequence I want to use. Can I change the promoter sequence or the enzyme?

I want to synthesize several genes with the promoter and terminator, I have chosen the enzymes I want to use and everything is ready to go. But when I requested the company to synthesize it, they...

07 July 2015 2,316 5 View