Osmar Rivera

3 Questions 3 Answers 0 Followers

Questions related from Osmar Rivera

How do I know if my drug is cytotoxic or just inhibits cell proliferation through an MTT?

I performed an MTT assay to see the effect of an inhibitor on AGS cells. I seeded them on Day 1, added the inhibitor on Day 2 and performed the MTT assay on Day 3 (left the inhibitor act for 24...

01 April 2022 4,328 3 View

Can I dissolve formazan crystals overnight?

I will perform MTT assays on cancer cells with 4 hours long treatments. Because of that, I would be finishing the MTTs too late, and my lab gets closed early. Can I leave the formazan crystals...

16 March 2022 163 4 View

What is the band of about 80 KDa I am finding in my casein zymography?

I am performing casein zymographies with conditioned media of cocultures of NKs cells and another cell line. In my zymography I always see a strong band of about 80 KDa. Does anyone know what...

09 October 2021 6,134 3 View