Oshin Misquitta

2 Questions 1 Answers 0 Followers

Questions related from Oshin Misquitta

Has anyone used the Invitrogen™ PureLink™ PCR Purification Kit for the clean up of aptamers ?

I am using the SELEX process to develop an aptamer. Has anyone used the Invitrogen™ PureLink™ PCR Purification Kit as a clean up step? My aptamer sequence is 80bp and the buffers in this kit only...

15 September 2022 903 0 View

Why is the DNA yield during the first round of SELEX so low ?

I started the conventional SELEX process; wherein my initial DNA library concentration was 10micro molar. At the end of the selection and after ethanol precipitation it was down to 11.67nM. How do...

04 August 2022 9,268 2 View