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Questions related from Orli Thau-Zuchman
Hi, I have two questions: 1. I am trying to improve my IHC staining in cerebellar OSC (penetration wise). Taking some z-sections, I was able to see (different staining/Abs') only around 40 um...
17 May 2016 3,058 2 View
I am due to start IHC on cerebellar organotypic slice culture (DIV21), and have some questions: 1. What is the recommended time frame for fixation? I saw, in different protocols, a range between...
24 February 2016 2,050 3 View
Can one prepare ahead and aliquot ACSF and keep it in -20C? The stock is made without CaCl.2H20 & D-Glucose, that will be added before bubbling and then using. (I am using the recipe: NaCL...
24 February 2016 2,449 3 View
