Orenda Zhao

2 Questions 1 Answers 0 Followers

Questions related from Orenda Zhao

How to test a protein in western blot from cell lysates without overexpression?

I want to show one protein (~70kDa) in one cancer cell line lysates using western blot, but no transient overexpression. Is that possible?? And I have use GENEVESTIGATOR to find out that the...

11 November 2019 6,225 3 View

Why Histone H3 and H4 form aggregation after biotinylation? How to avoid it?

Histone H3 and H4 were connected with 1 or 2 biotin through a short linker. And then they were stained to a dye through a streptavidin. Just after the biotinylation, the standard SDS-PAGE gel...

02 February 2019 8,530 3 View