How can I separate urea from a solution of urea/inorganic salt using a mixed-mode (Reversed phase and HILIC) HPLC column?

I am trying to separate urea in a solution of 2 wt% urea and 10 wt% inorganic salt. The current mobile phase is 95/5 ACN/Ammonium acetate at pH of 4.5 and the detectors are ELSD and UV. The tail...

10 January 2022 9,938 0 View