Ondrej Halgas

3 Questions 3 Answers 0 Followers

Questions related from Ondrej Halgas

What are proper cell and antigen concentrations for investigating cytokine secretion on RA PBMC cells after collagen stimulation?

I would like to analyze cytokines secreted by arthritic PBMCs (human) after challenging them with collagen. Is there a particular cell concentration and collagen concentration you would...

21 October 2024 3,713 0 View

Multiple protein sequence alignment that shows dots for identities?

Hi, Does anybody know what website can do multiple protein sequence alignment of highly similar sequences that would hide identical amino acids and show them only as e.g. dots - basically this...

08 March 2019 5,019 4 View

How do I decrease protein expression from pETDuet vector?

Hello everyone, I´m expressing Fab in E.coli - I´m using for this pETDuet vector (vector for two genes) - Fab part of heavy chain is cloned in 1st MCS, light chain in the 2nd MCS. This strategy...

09 February 2015 8,161 3 View