Omar Torres-Quesada

3 Questions 2 Answers 0 Followers

Questions related from Omar Torres-Quesada

Does somebody have experience with the cell line H1666 (lung adenocarcinoma)? Do they grow in RPMI-1640 media?

I am trying to thaw the cell line H1666 from ATCC. It is recommended to use DMEM F12 supplemented with lot of things (EGF, insulin, etc). Is the RPMI 1640 media also suitable? Thank you in advance

28 November 2018 5,776 2 View

Does somebody have a good and easy protocol to prepare samples from arthritic Knee condyles to Mass-Spectometry Analyses?

We want to sent to MS-analyses human arthritis knee cartilages to identify proteins from several parts. We would need a easy and good protocol to isolate proteins for MS. Thank you in advance!

28 November 2018 3,214 0 View

Artifact PCR band in the iCLIP cDNA library?

Hi! I am performing iCLIP experiments and at the last step, when I am trying to amplify my cDNA library with Solexa primers I get a band around 130 bp in my samples and also in the negative...

24 April 2018 2,526 2 View