Omar Santín Martínez

3 Questions 12 Answers 0 Followers

Questions related from Omar Santín Martínez

Monitor conformational changes in a protein influenced by pH?

I work with a protein that apparently likes acidic pH and I suspect that it do change its structural conformational when I change the pH. Apart from crystallography, NMR, circular dichroism, gel...

20 March 2020 6,388 3 View

Can Sepharose-Nanobody-(GFP) columns be regenerated after a GFP-tagged protein purification?

I am going to purify several proteins by using GFP-nanobodies linked to a sepharose column since my proteins have a GFP taq. Since I have not experience with this protocol, I would like to solve...

21 November 2018 1,732 1 View

Does all the Baculovirus Bac-to-Bac-like systems share donor plasmid compatibility?

I am going to clone several protein to express in Baculovirus. I am clonning them using one plasmid that I know is compatible with the Bac-to-Bac system that is being used in the lab, (since is...

26 October 2018 1,999 1 View