Omar Melo

4 Questions 2 Answers 0 Followers

Questions related from Omar Melo

Using multimaping reads or unique reads on featurecounts?

I'm working with Rat transcriptome (mRNA) using HISAT as aligner and featurecounts (subread) to count reads using BAM files from HISAT. Featurecounts has the possibility to count onli unique reads...

27 March 2023 8,447 1 View

Are RPA assays as tolerant of inhibitors as LAMP assays?

I want to couple RPA or LAMP assays to a non-rigorous DNA extraction method (as heat and spin method, or another), so I want to know is RPA assays are as tolerant of inhibitors as LAMP assays.

22 September 2020 7,023 2 View

Could someone recommend me a ultracentrifugation-free AAV viral vector purification protocol?

Someone who has tried some ultracentrifugation-free AAV viral vector purification protocol could recommend me a protocol to obtain high titers and purity of AAVs. I would really appreciate any help.

15 September 2019 8,215 4 View

Is it mandatory to remove primers and barcodes from reads before starting the analyzes in qiime 2?

Hello everyone, I have pair-end reads (2x300) from V4 16S region (515F 5′-GTGCCAGCMGCCGCGGTAA and 806R- 5′-GGACTACVSGGGTATCTAAT). I have 5 samples and 2 reads in fastq format (R1 and R2) for each...

29 October 2018 6,282 6 View