Olga Kozhar

3 Questions 12 Answers 0 Followers

Questions related from Olga Kozhar

How can I get pure DNA with CTAB extraction DNA protocol from fungal mycelium (Botrytis cinerea)?

I am trying to extract DNA with CTAB from fungal fresh-frozen mycelium (Botrytis) and ran into the problem that my DNA doesn't look clean on gel when I am trying to quantify it (picture is...

10 October 2015 5,020 10 View

How can I isolate Botrytis cinerea from asymptomatic raspberry or blueberry fruit?

I am working with Botrytis on small fruit crops and got a problem this year that I cannot find any symptomatic fruit on the fields because of very dry weather this spring and summer. So now I am...

07 July 2015 1,002 19 View

Allele-specific PCR for haploid organisms?

I am working with haploid ascomycete fungus and I am currently looking for a method of point mutation detection in a target gene of the organism. Based on the preliminary results of sanger...

01 January 1970 1,215 3 View