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Questions related from Olga Kudryavtseva
I run a Mg2+ dosis-response curve on phenylephrine-precontracted mouse aorta that should relax the aorta in a concentration-dependent manner (7 concentrations). I want to calculate EC50 of these...
04 August 2021 9,981 2 View
Hello everybody, I have to use apamin in vitro for experiments on isolated blood vessels. I am a bit unsure on how to dissolve/store it. Is it ok in water? I remember on my previous work place we...
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I have a basic IHC question, there is Tris-HCl buffer in the protocol I had gotten. How exactly to make it? There is also neither molarity nor pH specifications...I've been told that usually it's...
08 August 2018 10,096 7 View
Dear everyone, I have a question about intracellular dye delivery by intophporesis. I have a setup consisting of microelectrode connected to an electric circuit where I measure the membrane...
20 October 2017 7,133 4 View
Hello everyone, I am quantifying Ca2+ waves in pericytes and I am interested in the number of waves in the quantification period and/or their frequency. The problem is that my quantification...
17 October 2016 5,583 5 View
Hello dear everyone, I have a quite trivial problem but I could not find the straightforward solution in the articles. I have an oscillatory Ca2+ signal in cells in form of Ca2+ waves. I need to...
01 June 2016 7,317 6 View
