Oleksandra Kepple

3 Questions 9 Answers 0 Followers

Questions related from Oleksandra Kepple

How do you synthesize a peptide?

Dear all, I need to synthetize a peptide for antibody production, since I had a hard time to purify one very stubborn protein. Please, help me to figure out the right strategy how synthesize a...

03 February 2022 3,809 5 View

What does it mean if the Ni-NTA resin has turned into a gel in the column?

Dear All , I was purifying the proteins, following ProBond™ Purification System protocol (denaturing conditions) and noticed that Ni-NTA resin turned into something like gel structure inside the...

03 December 2021 6,192 3 View

What is the most effective way to elute a his tagged protein from the beads?

Dear All, I'm purifying some HIS tag protein in denaturing conditions and it seems that it does not elute from the beads efficiently (checked the gel).. Do you have any suggestions what is the...

25 October 2021 4,963 16 View