6 Questions 12 Answers 0 Followers
Questions related from Oki Jung
Hi. I am on experiment with proteasome inhibitor ( MG132 ) on A549 lung cancer cells. I treated 10uM of MG132 for 4, 24, 36 and 48hrs. However, it seems not to work. cells were less dead even...
03 March 2018 3,827 2 View
Hi I am trying immunoprecipitation by protein G. I tagged antibody( Rabbit) and I western blot using interesting protein antibody(mouse) I got a result lower picture. but it looks a little...
02 February 2017 5,885 1 View
hi i want to make stable cell. i use A549. i tried to do transfection on plasmid. but it is difficult to make it. so i tried to make linear plasmid before transfection. but i am not sure i have to...
08 August 2016 4,963 3 View
I am wondering if streptavidin or avidin can bind PCR tubes or a 96-well plate. I saw many products of 96-well plates coated streptavidin for ELISA. For my experiment using the ELISA sandwich...
10 October 2015 6,301 7 View
i just figure out that when i experiment transfection with tgfbeta induced EMT, what step i have to do at first. otherwise, can i do at the same time?
07 July 2015 680 0 View
i am trying to make 3KCl solution for storage buffer of ph meter. but the powder of KCl doesn't solve in water, i tried to stir it on hot water and do autoclave to increase high pressure and...
04 April 2015 9,702 13 View
