Nur Shafini Che Ahmad

3 Questions 2 Answers 0 Followers

Questions related from Nur Shafini Che Ahmad

Is HEPES concentration affect the cell line growth?

Hi, I am going to culture Raji B cells, I mistakenly ordered RMPI with 25mM of HEPES but recommended media by ATCC only suggested 10mM. is this will affect the cell growth? thank you.

11 November 2016 9,204 1 View

What is the best way to cryo caco-2 for cryo stocks?

Hi I just received a vial of Caco2 from ATCC. I am going to make stocks of it for next time uses. Once i revived the cells from the ATCC i found out that the cells have difficulty to attach to the...

11 November 2016 4,150 2 View

Is it possible to synthesise RNA from a known genomic sequence?

i am going to make an invisible virus to become visible. all i have right now is its genomic sequence from a Genbank. this virus is non culturable as currently there is no suitable cell culture...

04 April 2016 1,309 2 View