Nur SENA Si̇vri̇

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Questions related from Nur SENA Si̇vri̇

What is the appropriate primer for qPCR from genomic DNA?

Hi, Using the Methylation Analysis Kit with qPCR, I will check the DNA for methylation. I already have a pair of methylated and unmethylated primers for this purpose. Only F and R primers...

22 November 2022 1,754 2 View

Is agarose gel electrophoresis required for the cDNA control process?

I extracted RNA from pellets of mammalian cell culture and turned it into cDNA. To determine the level of gene expression, I'll use qPCR. Should I first run the cDNAs through agarose gel...

11 October 2022 7,608 4 View

How to keep cell pellet for further DNA isolation?

I would like to isolate both DNA and RNA from the same cells (mda-mb-231) after some treatments in culture. I will use different kits for each isolation, I will primarily isolate RNA for QPCR, but...

03 June 2022 8,154 1 View