Nuoc Non Tran

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Effects of cabinet UV lamp on then DNA contained in Eppendorf tube?

Hi everyone, I just wonder that I combined the DNA mixture with Lipofectamine in the Eppendorf tube, then I stand it at room temperature for 20-30 min (as protocol) in the clean bench of cell...

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How can I improve the quality of RNA? Can I use this RNA for Reverse Transcription and Realtime PCR?

Hi. I extracted RNA from soleus muscle (7-10mg) in mouse by using Quiazol reagents. And another method was that I extracted RNA by RNAeasy kit. The result showed that total RNA was 6 ug and 2ug;...

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Transfection using PEI for overexpression DNA in SY5Y and 293T cells.

I also have question involved with transfection using PEI. In my experiment, I try to transfect my DNA to SY5Y and 293T cells using PEI. In 12-well plate, I used 1.6ug DNA in 100ul OptiMEM mix...

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