Problems with COI amplification using Illumina overhanging adapter for eDNA samples, could it be that the bands are not the target region?

My PhD student has been working with eDNA samples working on 2 genes namely COI (Leray's-313bp) and the MiFish-12S primers. He is using Illumina overhanging adapter with specific primers to...

29 October 2018 4,189 3 View