Noha M. Hosny

4 Questions 7 Answers 0 Followers

Questions related from Noha M. Hosny

How can I decrease high pressure of c18 column?

I have a c18 column with a high pressure (2400 psi at flow rate 1 ml/min, acetonitrile:water 50:50). 1_ we use solvents for HPLC and were filtered by nylon paper. 2_ we tried 100% ACN and pressure...

10 May 2018 775 16 View

How can I separate drugs with carboxylic and amide groups by using HPLC?

I tried c18 and platinum columns also ion pair and B_CD were used, no separation was observed. Mobile phase , 90:10 v/v aqueous to organic solvent with and without buffer were not effective.

20 December 2017 651 2 View

How can i differentiate between uric acid and sodium monourate crystals either physically or chemically?

I have prepared uric acid sodium salt by using uric acid and sodium hydroxide. I want to make sure about what i have prepared.

06 May 2017 5,249 2 View

How can I prevent peak tailing in HPLC?

I am using acetonitrile and water as a mobile system and C18 column for separation of some drugs, but the obtained peaks were broad and tailed. How can I improve the shape of these peaks?

27 November 2013 873 29 View