7 Questions 11 Answers 0 Followers
Questions related from Nithipong Mapratiep
I try to investigate the inhibitory effects of six plant extracts on the virus infection but I'm not quite understand about IC50, CC50 and seletivity index (SI). Why do I have to measure it? Can I...
04 April 2015 2,090 11 View
I got the peptide solution (dissolved in sterile distilled water) in a concentration of 5 mg/ml from another lab and keep it in -80C. I want to dilute it into 100 ug/ml but I don't know which one...
03 March 2015 4,767 5 View
I try to dissolved three kind of plant extracts in 100% DMSO before I test them with the cell lines. The methyl extract is easily dissolved, but the hexane and ethyl acetate extracts are poor. I...
08 August 2014 6,744 8 View
I'm doing a growth curve for cells, but most of them started to die in 2 days after seeding into the 12 well plate. I try to infect them with the virus also, but the replication kinetics are not...
06 June 2014 6,121 9 View
I want to prepare a stock solution (100 mg/ml) from plant extract. How to do that? The extract have to dissolve in DMSO and the final concentration of DMSO have to be 1-2% in the cell culture...
04 April 2014 7,730 11 View
I'll start working with THP-1 cells but I don't know how to passage it efficiently. Someone told me that, this cell will adhere to the surface so I have to remove the old media and incubate them...
02 February 2014 2,979 7 View
Is it contaminated? What should I do to eliminate it? Are there any effects on the experiment?
01 January 2014 4,732 5 View
