I couldn't separate my methanol fraction by UV-HPLC. I've tried with C18 and water-methanol solvents but it still a single peak. Any thoughts why?

I've isolate secondary metabolites from marine fungus by extracting and fractioning the culture media of the fungus. But when i tried to subfractioning my methanol fraction, the fraction wouldn't...

18 May 2019 7,993 5 View