Nisha Sinha

4 Questions 4 Answers 0 Followers

Questions related from Nisha Sinha

Should the Ct value of the reference gene be less than the target gene in miRNA RT-PCR?

I have performed RT-PCR for exosomal miRNA. For normalisation I've used U6. But Ct value of the target gene is less than the normalised gene (U6). U6 is giving a Ct value of approx 32 whereas...

03 March 2015 3,786 6 View

Is it possible to generate non proteinuric diabetic cell culture model?

I would like to study diabetics with protein and diabetics without protein at molecular level in cell culture model. Is it possible to have non proteinuric diabetic in vitro model. Kindly suggest.

01 January 2015 4,290 1 View

What is the minimum number of samples for miRNA microarray expression profiling to achieve significant data? Is it 2 or 3 in each group?

We need to perform miRNA expression profiling in our samples using affymetrix platform. Do the samples need to be done as technical replicates or biological replicates?

01 January 2014 7,774 5 View

To look for miRNA in the sample I first did real time PCR for control U6. But got a high Ct value of 35 even after diluting cDNA. Any suggestions?

04 April 2013 2,685 0 View