Nirmani Wickramasinghe

3 Questions 4 Answers 0 Followers

Questions related from Nirmani Wickramasinghe

How can I detect Eukaryotic RNA contamination in Prokaryotic RNA samples using qPCR?

Hi, I am trying to detect Eukaryotic RNA contamination in my bacterial RNA samples. I plan to sequence the bacterial RNA to get a transcriptomic profile. I am aware that qPCR can show if there is...

03 March 2019 5,552 2 View

What is the best medium to isolate fluorescent pigment producing pseudomonads? Are there any such commercial media available ?

I am searching a method to differentiate Pseudomonas spp from environmental samples. I can use CFC agar to isolate all the Pseudomonas spp but is there a specific media to isolate fluorescent...

02 February 2018 2,269 5 View

Are there any fluorescent stains that are specific to bacterial cells that would not stain eukariotic cells?

I am trying to image and quantify bacterial cells on muscles using confocal laser scanning microscope. But I am having trouble quantifying the bacteria as the stains that I used ( live/dead...

10 October 2017 2,506 8 View