Nimerta Kumari

3 Questions 7 Answers 0 Followers

Questions related from Nimerta Kumari

I'm trying to overexpress two peptides 8 KDa and 7 KDa in E coli 2DE3 Rosetta using pET28a+ vector, but unable to express my proteins?

I am trying to over express two peptides 8 K Da and 7 K Da, in E coli 2DE3 Rosetta, using pET28a+ vector. I used LB media, induced the cell at 0.5mM and 1mM IPTG, using the OD 0.8. I induced the...

09 September 2013 867 14 View

I have small peptide only 50 aa, Mol. wt. of 5734. Does it can induce an immune response in mice? As litrature says < 6K Da could not be an immunogen?

I am trying to raise the polyclonal antibodies against my peptides in mice or rabbit but its size is very small, only 5.7 K Da. But before going through it I want to ask if anyone has experience...

08 August 2013 7,632 4 View

What is easiest way to dtermine biofilm cfu/ml after sensitivity testing?

Scraping of biofilm cells from the side walls of flat bottom well plate is desirable or not?

01 January 2013 1,019 2 View