Nima Mohaghegh

2 Questions 9 Answers 0 Followers

Questions related from Nima Mohaghegh

Has anyone cultured THP-1 cells in Shaker-flasks?

I wonder if anyone has cultured THP-1 cells in shaker-flasks? If so, what is the appropriate shaking rate? And if you recommend any anti-foaming reagent being added to RPMI? Or, do you recommend...

06 June 2016 6,377 3 View

Which cell lines and TLR-ligands do you recommend for IRF5 and/or IRF7 activation -in terms of nuclear translocation and/or phosphorylation?

It is a while that I'm trying to recapitulate the literature for IRF5 and/or IRF7 nuclear translocation and/or phosphorylation upon TLR3/ 4/ 7 stimulation in undifferentiated or PMA-differentiated...

12 December 2015 7,358 2 View