Nilanjana Basu

5 Questions 14 Answers 0 Followers

Questions related from Nilanjana Basu

Are these MCF10A cells healthy?

Hello! I have revived the MCF10A cells after a long time. They seemed fine after revival but after the first passage, I find these black dots floating in the suspension. Earlier I read that over...

18 August 2021 818 4 View

Does anyone use M-per lysis buffer for protein extraction?

Hi I want to know the protocol of using M-per lysis buffer for protein extraction. I have came across many protocols which says to use it alone. Some says use it in combination with Protease...

20 July 2021 4,257 4 View

What are the reasons of variation of CT values of the control and test in the housekeeping gene ?

I have been working with the same primers and cDNA, in which earlier the CT values were similar with 0.5 cycles in test and control. In the third occasion the CT values of test and control in the...

27 April 2021 7,864 3 View

Annexin V FITC Propidium Iodide Assay - Why Apoptotic population is not visible?

Hello! I have been using Annexin V FITC Propidium Iodide from a commercial kit and got similar results twice. Unfortunately, for the third experiment, the PI of the kit got exhausted. Someone...

03 November 2020 6,589 4 View

Which is an ideal in vitro model to elicit the effect of a drug in cytokine storm?

Hello everyone! Trust all are doing good research in this COVID-19 pandemic. My question is to find out an ideal in vitro model to study cytokine storm. Also if one needs to elicit effects of a...

30 June 2020 4,224 3 View