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I am curious how it actually works with carboxyl group that attach to the paramagnetic beads and the covalent bonding of them with DNA when the purification of DNA using paramagnetic beads is...
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I'm trying to insert an His-tag in my plasmid using fusion PCR, but I'm not sure how it actually works. Is the primer like having a long tail of our fragment that we want to overlap with our sample?
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