Nikunj Tyagi

4 Questions 2 Answers 0 Followers

Questions related from Nikunj Tyagi

Why does religation problem persists in Vector backbone while digesting with two different restriction enzymes?

Our aim is to transfer gene from bacterial plasmid(pET22b) into mammalian expression vector pACGFP1-N1, i have decided restriction sites available in pACGFP1-N1 vector MCS but while doing...

25 January 2023 479 3 View

What are the tools to analyze protein network other than STRING of more then 2000 proteins?

We have a protein profile of around 4000 proteins, and we tried Cytoscape since STRING has limitation of 2000 maximum , Cytoscape slowed down the system and taking more time, i also tried GEPHI...

15 February 2022 2,118 0 View

I have a list of proteins and there Sequences in my MS/MS data , now i want to verify the Glycoproteins from this list, any tools recommendation?

Proteins were isolated from Buffalo Gravid uteri cotyledons, Glycoproteins were enriched by using different lectins and the sample was further sent for LC-MS/MS. now i got the list of proteins...

17 September 2021 7,332 2 View

What is the good Molprobity score for a protein homology model??

I build a homology model for a protein which show 44% sequence identity with its template and molprobity score is 1.59

28 December 2019 3,451 4 View