Nikolai N. Sluchanko

4 Questions 9 Answers 0 Followers

Questions related from Nikolai N. Sluchanko

Safe concentrations of CHAPS for performing size-exclusion chromatography of a CHAPS-solubilized protein?

A chromophorylated protein is dissolved from a washed pellet fraction by 2% CHAPS, which preserved the color of the protein. Anything against subjecting this soluble prep to SEC in the presence of...

27 February 2020 8,338 5 View

How to export chromatograms and spectra from diode array detector data?

The DAD data are collected into a .run file during an HPLC run followed by a Varian Prostar 335 detector. The file cannot be merely opened via a notepad and the question is how to extract all the...

05 April 2019 9,781 3 View

An all-alpha helical protein changes a 208/222 nm ratio on its far UV CD spectrum. Any suggestions?

Some modification changes the CD spectrum of a small helical protein so that the 208nm band becomes more pronounced relative to the 222 band. Are there any reliable interpretations? Paper...

05 July 2017 5,488 1 View

Why can a protein elute from a SEC column (@150 mM NaCl) later than expected for its monomer?

Theoretical mass is 25 kDa, SDS-PAGE shows 26 kDa, no presence of degradation products. The SEC profile shows the major, very symmetrical peak at ~20 kDa. Why could this be? Unspecific interaction...

29 August 2016 2,138 16 View