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Questions related from Nikolai N. Sluchanko
A chromophorylated protein is dissolved from a washed pellet fraction by 2% CHAPS, which preserved the color of the protein. Anything against subjecting this soluble prep to SEC in the presence of...
27 February 2020 8,423 5 View
The DAD data are collected into a .run file during an HPLC run followed by a Varian Prostar 335 detector. The file cannot be merely opened via a notepad and the question is how to extract all the...
05 April 2019 9,857 3 View
Some modification changes the CD spectrum of a small helical protein so that the 208nm band becomes more pronounced relative to the 222 band. Are there any reliable interpretations? Paper...
05 July 2017 5,547 1 View
Theoretical mass is 25 kDa, SDS-PAGE shows 26 kDa, no presence of degradation products. The SEC profile shows the major, very symmetrical peak at ~20 kDa. Why could this be? Unspecific interaction...
29 August 2016 2,210 16 View
The his tag is of 12 His residues at the C-terminus of the protein without a linker. So, what should be considered besides points of the typical protocol? Higher imidazole concentration of the...
02 February 2015 1,547 4 View
The idea is to visualize high-order oligomers of a protein which can self-associate but normally exists as small oligomers. Is it OK to use native-PAGE and gels containing TMAO? Which TMAO...
12 April 2014 8,216 0 View
