Nikki Miguel

3 Questions 2 Answers 0 Followers

Questions related from Nikki Miguel

Why should skim-milk be used for blocking buffer in western blots?

I have just performed my first western blot and none of my targeted proteins were detected. The only thing visible on the membrane was the prestained ladder. Someone suggested that my blocking...

22 August 2018 6,075 4 View

Why would LB-low salt medium be prefereable to normal LB medium?

I've come across a protocol that says to use low salt LB broth (10g/L tryptone, 5g/L NaCl, 5g/L yeast extract) for bacterial growth curves. Why would it be preferable to use a low salt medium...

19 February 2018 2,212 2 View

Why is the deletion of bcsQ using lambda red recombination unsuccessful?

I have been unsuccessful in knocking out bcsQ in Pectobacterium carotovorum ssp. brasiliense using a three-step PCR method. Plasmids used: pKD4 and pKD46 bcsQ is MinD homologue part of the...

18 July 2016 1,582 2 View