Nikhil Ratna

4 Questions 2 Answers 0 Followers

Questions related from Nikhil Ratna

Can we snap freeze tissues for paraffin embedding later?

Hi all, I wanted to know if snap freezing the live tissue is helpful when one is aiming for paraffin embedding and sectioning (at room temp) later. Will the tissue gets damaged when it thaws? My...

09 December 2021 9,272 4 View

How long does the drosophila tissues (imaginal discs, muscles, brains) be alive in PBS/TBS at RT and on ice?

Often I am thinking about the viability of the tissues in PBS after dissecting before fixing or staining to know how it affects my readouts. especially for live staining, tissues need to be alive...

20 November 2021 2,087 0 View

Which salt of paraquat is commonly used for drosophila mitochondrial studies?

Hi all, I am trying to find which salt of paraquat is used in drosophila studies where it is given by mixing in food. objective is to stress the mitochondria for an experiment. It will be of...

27 October 2021 4,190 1 View

Troubleshooting sample prep protocols for making nuclei suspension from mouse/human brain tissue for snRNAseq

Hi all, I am starting this thread to connect and share resources/knowledge on sample prep protocols for extracting nuclei to performing snRNA seq experiments. The main issue with this is that...

01 January 1970 9,583 0 View