Nikhil Ratna

2 Questions 2 Answers 0 Followers

Questions related from Nikhil Ratna

Can we snap freeze tissues for paraffin embedding later?

Hi all, I wanted to know if snap freezing the live tissue is helpful when one is aiming for paraffin embedding and sectioning (at room temp) later. Will the tissue gets damaged when it thaws? My...

09 December 2021 9,148 4 View

How long does the drosophila tissues (imaginal discs, muscles, brains) be alive in PBS/TBS at RT and on ice?

Often I am thinking about the viability of the tissues in PBS after dissecting before fixing or staining to know how it affects my readouts. especially for live staining, tissues need to be alive...

20 November 2021 2,045 0 View