Nihan Şahin

4 Questions 9 Answers 0 Followers

Questions related from Nihan Şahin

Why polyacrylamide gel doesn't conduct electricty?

I'm doing everything properly according to standard protocol but still my gel doesn't conduct electricity. So why? I can't solve it.

04 April 2016 6,532 5 View

An economic method for the fluorescent labeling of PCR fragments?

I have a trouble with my pcr reactions and i want to ask you where is my fault.I designed six primer pairs according to Schuelke's M13 methods but I did not get any reaction.One of my primers...

12 December 2014 6,929 6 View

How should I calculate a touchdown PCR reaction with a low dna quantity?

I have an average of 1.5 ul/ml total dna quantity on my vine dna samples. In this case, how should I calculate a touchdown PCR reaction for 20 - 25ul?

09 September 2014 7,046 4 View

How many primers?

I want to make a local molecular characterization of F. vesca genome. How can I decide of how many primers this requires?

02 February 2014 426 5 View