Nidhi Sharma

3 Questions 7 Answers 0 Followers

Questions related from Nidhi Sharma

How can I avoid aniline interference while quantifying the protein (Ab) immobilized using GlycoLinkTM Immobilization kit?

Hello everyone, I am using GlycoLinkTM Immobilization Kit (Thermo scientific 88941) for immobilizing our antibody. However, while using the kit we faced the following problems: According to the...

18 November 2015 9,539 2 View

Is 40% identity between the query sequence and the subject good enough for selecting antibodies?

I am looking for an antibody for HSP beta1 (small heat shock protein in Crassostrea gigas EKC40046.1). In mouse HSP beta1 is also known as hsp27, hsp25 (UNIPROT kb/SWISS-PROT: P14602.3). I aligned...

27 November 2013 8,932 2 View

Can any one tell me the best way to purify cDNA? Is it by column or precipitation with sodium acetate and ethanol?

I understand that it is not necessary to purify cDNA for real time PCR (as I had some good results previously) but still I want to be really sure.

02 October 2013 2,339 10 View