Nicolò Taricco

4 Questions 3 Answers 0 Followers

Questions related from Nicolò Taricco

[CHO-K1 cell line] What UCSC reference genome to use to design primers?

Hello! I would like to design primers for a set of genes involved in apoptosis (p53, BAX, BCL2, etc) using chinese hamster CHO-K1 cell line. However, when I browse UCSC looking for the genes'...

05 May 2018 4,319 3 View

Is there a method to check for RNA integrity/quality that does not require to run an agarose gel nor to use Bioanalyzers?

Hi everybody, I would like to assess the quality of my RNA before performing further analyses (RT-qPCR) but I do not have access to Bioanalyzers like the Experion or Agilent 2100 Bioanalyzer. I...

07 February 2018 1,003 2 View

How much time should I wait for a broad epigenetic response after Valproic Acid treatment?

Hi, I would like to treat chicks with valproic acid, an inhibitor of HDAC1 and other HDACs, for studying specific regions of the brain. However, I am not sure about the end point at which the...

07 February 2018 7,494 2 View

Could someone tell me if Glycoblue (ThermoFisher) can affect Nanodrop readings of RNA samples?

I use Glycoblue to dye my RNA pellets deriving from cytosolic or nuclear RNA (concentrations ranging from 150 to 600 ng/uL) and everything is pretty fine with the absorbance ratios from...

04 May 2017 6,089 4 View