Nicoletta Plotegher

3 Questions 11 Answers 0 Followers

Questions related from Nicoletta Plotegher

Have you experienced any problems in solubilizing 4-Methylumbelliferyl β-D-glucopyranoside to perform glucocerebrosidase activity assay?

I performed activity assays on cell lysates following the protocol described in Osellame et al. 2013. I purchased 4-Methylumbelliferyl β-D-glucopyranoside from Sigma (M3633) and in the datasheed...

12 December 2015 4,400 10 View

Does anyone know an estimate of alpha-synuclein protein concentration in neurons in physiological conditions?

Please provide references to papers in which alpha-synuclein concentration is estimated or to papers in which a suitable method to quantify protein concentration in neurons is applied.

01 January 2014 3,994 7 View

Does anyone have an effective protocol to separate cytoplasm from nuclei in cultured cells to perform western blot analysis?

I'm doing some preliminary experiments related to the traslocation of a protein to the nucleus under stress conditions, but I've never done the separation of the nuclei from the cytoplasm. I need...

05 May 2013 260 8 View