Nicolas Tavernier

6 Questions 14 Answers 0 Followers

Questions related from Nicolas Tavernier

Can ATP bind to Q column and be eluted by salt?

Hi, Can ATP bind to Q column at pH=7.5 and be eluted by NaCl salt gradient? And can ATP be detected at 280 nm? I washed my Q column with the binding buffer containing 5mM ATP/3mM MgCl2 to get rid...

11 November 2016 4,127 6 View

How do I assess the DNA contamination of a protein preparation?

Can we trust the 260/280 ratio? Do you have an alternative method to propose? Thank you. Nt

11 November 2015 7,685 4 View

Difference and compatibility between ColE1, pBR322 and pUC plasmid origins?

Hi all, Could you please explain what is the difference between the replication origin pBR322 and ColE1 ? If you have look on that vector databases:...

10 October 2015 10,444 11 View

Have you ever try to perform a PCR from a Baculovirus solution?

I would like to amplify the cDNA of my protein of interest from the Baculovirus suspension. Actually, I would like to be sure that a specific mutation is well present.Any recommendations?

09 September 2014 4,082 4 View

What is the basis of structural biology?

Do you know any good reviews or books related to the basis of structural biology for a beginner in the field? Thank you in advance for your help. Best.

03 March 2014 10,033 8 View

What concentration of a CDK1 inhibitor should I use knowing the Ki?

I’m looking for advice regarding inhibition of kinases with specific chemical inhibitors. For instance, cdk1/cyclin B with the inhibitor RO-3306. In Vassilev et al, PNAS, 2006, it’s indicated that...

01 January 2013 5,046 6 View