6 Questions 14 Answers 0 Followers
Questions related from Nicolas Tavernier
Hi, Can ATP bind to Q column at pH=7.5 and be eluted by NaCl salt gradient? And can ATP be detected at 280 nm? I washed my Q column with the binding buffer containing 5mM ATP/3mM MgCl2 to get rid...
11 November 2016 4,129 6 View
Can we trust the 260/280 ratio? Do you have an alternative method to propose? Thank you. Nt
11 November 2015 7,687 4 View
Hi all, Could you please explain what is the difference between the replication origin pBR322 and ColE1 ? If you have look on that vector databases:...
10 October 2015 10,448 11 View
I would like to amplify the cDNA of my protein of interest from the Baculovirus suspension. Actually, I would like to be sure that a specific mutation is well present.Any recommendations?
09 September 2014 4,084 4 View
Do you know any good reviews or books related to the basis of structural biology for a beginner in the field? Thank you in advance for your help. Best.
03 March 2014 10,035 8 View
I’m looking for advice regarding inhibition of kinases with specific chemical inhibitors. For instance, cdk1/cyclin B with the inhibitor RO-3306. In Vassilev et al, PNAS, 2006, it’s indicated that...
01 January 2013 5,048 6 View