Nicola Faria

3 Questions 4 Answers 0 Followers

Questions related from Nicola Faria

How do I confirm at genomic level that mytomycin C has induced the prophage ?

I have induced the prophage from the host bacteria using mitomycin C. The prophage particle was concentrated via Cscl density gradient ultracentrifugation . The structure of bacteriophage was...

22 February 2018 6,938 4 View

How to propagate & concentrate the prophage after induction with mitomycin C?

Prophage Induction with mitomycin C (1ug/ml) gave me reduction in bacterial turbidity measured at OD 600 nm. A spot test of the lysate had given a noticeable clearance. But there no plaque...

13 February 2017 5,202 4 View

Does PEG interfere with the TEM analysis of phage?

Can I precipitate the phage using PEG8000 and go for TEM analysis directly? or Is it necessary that I concentrate with CsCl Density gradient ultra centrifugation?. Do PEG 8000 interfere during...

13 February 2017 2,907 1 View