Nick Schaum

3 Questions 7 Answers 0 Followers

Questions related from Nick Schaum

Why do my amplification curves appear dampened with the Fluidigm gene expression array?

I am using the 96.96 Fluidigm gene expression array with samples from various mouse tissues (not single cell), as well as a set of purchased fetal cDNA. In the attached image, the group of...

08 August 2015 3,450 2 View

Do I need two sets of primers for each gene for the Fluidigm gene expression array?

I have conflicting recommendations from Fluidigm tech support and users as to whether it is worth it to run each gene with two sets of primers. I am running a 96.96 gene expression array on...

04 April 2015 2,623 5 View

Are bioanalyzer results reproducible?

I have heard that there can be a good deal of variability in reported RNA concentration and RIN for repeated measures of the same sample. In one case, the reported concentration was 10-fold...

04 April 2015 4,735 2 View