Nicholas Weiler

3 Questions 6 Answers 0 Followers

Questions related from Nicholas Weiler

What cortical neurons express vGluT2?

Many papers use differential expression of vGluT1 and vGluT2 vesicular glutamate transporter isoforms to identify intracortical vs. thalamocortical synapses, respectively. However, many of the...

04 April 2013 3,004 17 View

What are the best methods for automated identification / quantification of cell density using DAPI (or similar) nuclear stain?

I'm looking for recommendations or citations for good computational methods for thresholding and counting cell bodies in fixed tissue samples based on a nuclear stain such as DAPI. Simple...

04 April 2012 5,148 6 View

Is RNAse-free H2O necessary / recommended for immunohistochemistry?

Clearly for in situ hybridization or other molecular biology techniques, RNAse-free water is a necessity. However, is there significant danger in using regular distilled / autoclaved water for...

03 March 2012 6,002 15 View