Nicholas Santangelo

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Questions related from Nicholas Santangelo

Does anyone have a good method for getting bubbles out of your mounts when you mount straight to slides from a cryostat?

We are mounting brain sections (20 microns) straight from cryostat to subbed slides. I was wondering if there was a good method out there for extracting bubbles that sometimes get under the mounts?

06 June 2014 8,164 8 View

Has anyone had an issue with floating mounts of brain tissue using Master Tech's Poly-L-Lysine subbed slides?

I mounted brain tissue for an ICC (typical protocol - cyrostat sectioning with OCT at -15, stored slides at 4 C until running the ICC..about a week or two). When we started the ICC, a few mounts...

06 June 2014 1,515 0 View